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Estimation of mitochondrial mass Mitochondrial mass was measured by MitoTracker dye as previously described 76

Known as the body's master antioxidant, Glutathione plays a critical role in protecting cells from oxidative stress, supporting immune function and aiding in detoxification

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As the C-termini of all zDHHCs except zDHHC24 face the cytosol, we hypothesized that the addition of a C-terminal KASH-domain might induce their targeting to the ONM (Fig