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Real-time PCR of mRNA levels cDNA was synthesized from total RNA (1 g) extracted from mouse BAT using TRIzol Reagent (Invitrogen, Tokyo, Japan)
The clinical implications of this are profound
Type 2 diabetes mellitus Nearly 10% of the US population is affected by type 2 diabetes mellitus and another 34% has impaired glucose control (prediabetes) that places them at high risk of developing type 2 diabetes (13)
For example, individuals exposed to ES exhibit an increased mitochondrial DNA copy number content in leukocytes [193] and in saliva [194] indicating that this effect might be widespread throughout the body and have its origin in childhood
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