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After cell lysis, cDNA synthesis was carried out (Maxima Reverse Transcriptase, Thermo Fisher), followed by PCR (2 Kapa Hotstart Ready mix, VWR, 15 cycles)

Sources Unleashing the power of retatrutide: A possible triumph over obesity and overweight: A correspondence PMC TripleHormone-Receptor Agonist Retatrutide for Obesity A Phase 2 Trial New England Journal of Medicine Type 2 Diabetes Treatment to Lower A1C Mounjaro (tirzepatide) (lilly.com) Zepbound (tirzepatide) Injection for Adults with Obesity or Excess Weight (lilly.com) NowPatient has taken all reasonable steps to ensure that all material is factually accurate, complete, and current