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These metabolites are involved in numerous biological processes within tumor cells, including transcription, translation, post-translational modification (PTM) of proteins, cell cycle regulation, and metabolism, thereby modulating tumor proliferation, migration, and drug resistance
In brief, 1 10 5 spleen Ter119+ cells were pelleted in PBS, resuspended in 30 l of Buffer CM and incubated at 24 C for 1 h, with occasional vortex
Importing peptides from international suppliers introduces additional variables
Avoid freeze/thaw cycles, which can degrade peptide structure
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