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After incubation with secondary antibodies (Thermo Fisher Scientific, A32766/A32790/A31572/A31570, 1:500), at room temperature for 2 h, samples were co-stained, when indicated, with either PNA (Vectorlabs, RL-1072, 1:1000) or WGA (Invitrogen, W32466, 1:1000) at 37 C for 1 h

GLP-1 receptor agonists are medications that mimic this hormone, amplifying its effects to produce meaningful, sustained weight loss

At low concentrations, they function as antioxidants, but at higher doses, they can promote the formation of free radicals, leading to oxidative stress and cellular damage

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