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Muscle tissue burns more calories at rest than fat, so losing muscle mass can slow your metabolic rate and make long-term weight maintenance harder after treatment ends

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Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]

Berberine basically tells your body to use glucose and energy more efficiently and burn more fat, Berger explains