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Samples were permeabilized, blocked (Dako, #X0909) and then incubated with rabbit anti-glucagon (Santa Cruz, 1:200 discontinued) and mouse anti-GLP-1 (736) amide (Abcam #ab26278, 1:200) antibodies
Quantitative real-time PCR (qRT-PCR) Total RNA was isolated from pancreatic tissues and cultured PSCs using TRIzol reagent (Beyotime)
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Immune cells generally express low GLP-1R, yet macrophages and T lymphocytes carry functional levels, suggesting an immunomodulatory axis [28,29,30]
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