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Biased genome-wide screening was also performed using the Calabrese CRISPR activation (CRISPRa) system in tandem with scRNA -seq following induction of ferroptosis
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This study established a high-efficiency DF-1 cells system for IBDV vaccine production by regulating glutathione metabolism, and underscored the importance of moderate gene expression regulation on the virus reproduction providing a way for rational and precise cell engineering
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