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After stimulation, cells were washed once with ice-cold PBS and collected in RIPA buffer (MilliporeSigma) supplemented with protease and phosphatase inhibitors (BioTools)
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These medications are administered via subcutaneous injection, with dosing schedules varying from daily to weekly depending on the specific formulation
Type 2 is our path to new treatments, frankly, especially for the complications, said Tuttle
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