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Microneedling pre-treatment creates transient aqueous pores that can increase hydrophilic peptide delivery substantially, making it the most evidence-adjacent delivery method, though still not formally studied for GHK-Cu in controlled human trials

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The relative gene expression was calculated as 2 CT ( TABLE 1 Gene Expression Assay that were used to determine gene expression

This balance supports beneficial vasodilation without the tissue damage associated with excessive nitric oxide production during inflammatory states