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This increased spend was comprised of costs associated with the inpatient and outpatient settings as well as for prescription medications [not including anti-obesity medications (AOMs)] 6

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The eluted sample was quickly neutralized by addition of 1/10 volume of 1 M Tris pH 8.0 and further polished on a size-exclusion chromatography column (Superdex 75, GE healthcare) pre-equilibrated with phosphate-buffered saline (PBS, pH 7.4)

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