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Measurements of urine volume, osmolality, and excretion Male C57BL/6J lean mice were housed in individual metabolic cages for 5 days to adapt to the environment
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A senescence -galactosidase assay was conducted using the senescence -galactosidase staining kit (Cell Signaling Technology, 9860) according to the manufacturers instructions, and then stained cells were imaged using a ZEISS Primovert inverted microscope (Carl Zeiss Microscopy)
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