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If this doesnt give a clear result, other tests, like an array comparative genomic hybridization (aCGH), might be needed
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The first PDZ domain of ZO-1 interacts with claudin proteins, the second domain interacts with other ZO proteins (ZO-1, -2 and -3), and the third domain interacts with JAM-A (Ulluwishewa et al., 2011)
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