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Immunoblotting Total cell lysates were collected in NP-40 lysis buffer (150 mM NaCl, 1% Nonidet P-40, 50 mM Tris, pH 8.0 and a protease inhibitor cocktail) and protein concentrations were determined by BCA assay (Bio-Rad Laboratories, Inc., Hercules, CA, USA)
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O tratamento da obesidade envolve mudanas de hbitos e acompanhamento multidisciplinar
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