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It is the confidence with which preliminary or mechanistic evidence is translated into clinical implication
Cells were seeded at a density of 1 10 4 cells/well in 96-well plates and incubated for 24 h at 37 C in a 5% CO 2 humidified atmosphere to allow adherence
Cell culture and cell Viability, transfection of cells with SiRNA The ATDC5 chondrocyte cell line (RCB Cat# RCB0565, RRID: CVCL_3894) was obtained from Genomeditech Biotechnology Co., Ltd
This way, I am clean and my muscles are relaxed and warm, which means the the injection is less painful or totally pain-free
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