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Cells were then blocked with 5% goat serum for 1 h at R.T and stained with 1300 dilution of anti-GST A4-4 polyclonal antibody overnight at 4C, followed by incubation with FITC-conjugated secondary antibody for 1 h at room temperature as described before [29]
Fibroblast Growth Factor 21 (FGF21) Protects against High Fat Diet Induced Inflammation and Islet Hyperplasia in Pancreas
After seeding in 96-well plates pre-coated with poly-L-lysine at a density of 100 L/well, the cells were pre-incubated at 37C and 5% CO 2 for 34 h to form a monolayer
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