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A microbiome-based random forest classifier was therefore constructed to predict the TMAO producer phenotype (AUROC = 0.81) which was then validated with an external cohort (AUROC = 0.80)

Rational combination therapies that simultaneously address multiple resistance pathways are likely to become increasingly important, particularly those integrating targeted agents, epigenetic modulators, metabolic inhibitors, immunotherapies, microbiome-directed interventions, and TME-reprogramming strategies

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