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2.7 G protein activation assay by Nb37 BRET A construct encoding SNAP-GLP-1R with a C-terminal nanoluciferase was generated in house by PCR cloning of the nanoLuciferase sequence from pcDNA3.1-ccdB-Nanoluc (a gift from Mikko Taipale
35 Notably, this protective effect appears to be dependent on the duration of treatment, with only a GLP-1RA treatment period exceeding 52 weeks being associated with decreased fracture risk
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