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What Success Looks Like Realistic expectations: 30-40% improvement in density
The model presented here is in agreement with others linear-based and discontinuous epitope predictions 29,30
Sulfating activities of SULT2B1a allozymes with pregnenolone The sulfating activities of purified SULT2B1a allozymes toward pregnenolone were experimentally determined and compared to the wild-type enzyme
mansoni TGR was amplified by PCR (forward primer 5-CTATTTGGCTAGACGTCTGT-3 and reverse primer 5-AATACAGTTTCCTTCCCGTT-3) and cloned into PCRII-TOPO vector (Invitrogen, The resulting construct was linearized using XhoI (for SP6 RNA polymerase transcription) and SacI (for T7 RNA polymerase transcription)
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