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Vitamin B12 is a water soluble vitamin, so it is pass through the urine, excess amounts have not been shown to cause any harm
Although these tests are easy to perform and can provide some basis for preliminary safety evaluation, their prediction accuracy is limited and may not be enough to accurately evaluate the risk of phototoxicity ( in vitro method for animal skin phototoxicity testing, 3T3 NRU-PT has the advantages of simple operation, low cost, good reproducibility and a short test period and has good correlation with in vivo experimental results (Seto et al., 2015)
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