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They also ran flow cytometry (Annexin V/PI staining) and western blot analysis to measure specific survival and death markers: Bcl-2, Bax, caspase-3, and PARP

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It was recently shown that antioxidants can prevent loss of HSCs self-renewal potential in several contexts, such as aging or after exposure to low doses of irradiation, suggesting that antioxidants can be used to maintain HSCs functional properties upon culture-induced stress [20, 21]