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D'alessandro, Universit Di Palermo, Palermo, Italy, (3)University of Palermo, (4)University of Florence, Italy, (5)University of Manchester Background: Insulin resistance is an early event in nonalcoholic fatty liver disease (NAFLD), but the molecular mechanisms underlying the reduced response to insulin are still elusive
Caspase activation and inhibition
The PCR product containing the polymorphism rs2070593 was digested with 7 U of Psp CI restriction enzyme (Sibenzyme, Novosibirsk, Russia) producing two fragments of 183 and 180 bp (these bands overlap each other at electrophoresis) in case of the G allele and leaving the A allele uncut (one fragment of 363 bp) on 2%-agarose gel
Why should I not shake the vial
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