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We first used HEK293T cells, which lack endogenous caspase-1, to identify off-target cleavage events against a clean background (Extended Data Fig

It incorporates key modifications, including 14E/17R mutations to stabilize an -helical segment via a salt bridge, multiple proline substitutions (25P, 28P, 29P) to reduce -sheet formation, and N-terminal C20 fatty diacid lipidation for reversible albumin binding (Kruse et al

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