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Inborn errors in cobalamin metabolism, such as cblC defects, can result in a range of neurological manifestations, including demyelination and neurotoxicity [98] and have even been linked to MS [99]
After TMB substrate solution is added, only those wells that contain Human OPG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color
If any product remains, it should be stored closed in the refrigerator and uses promptly
However, technology-based delivery (through the internet, remote communication technologies, or deliverables like DVDs) may improve access to the treatment and may benefit patients reluctant to engage in face-to-face therapy, or unable to conveniently meet with a trained therapist
