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Description

For recycling, cells were incubated with agonists at 37 C for 15 min, followed by endocytosis arrest at 4 C, surface label removal as above, 30 min incubation at 37 C with 10 M exendin(9-39) to prevent further internalization, and a second round of surface label removal at 4 C

It can sit there indefinitely

This allows time for potential recurrence to manifest and be detected

For example, is the irritability due to the medication itself, or is it a result of changes in eating habits, blood sugar levels, or general lifestyle adjustments