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GHK-Cu's copper component is the main reason cycles are time-limited

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Evaluation of aflatoxin B1 mutagenesis: addition of glutathione and [PMID: 3107976] Laboratory literature summary: Evaluation of aflatoxin B1 mutagenesis: addition of glutathione and glutathione-S-transferase to the Salmonella mutagenicity assay

Critically, never shake the vial after adding water