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Add 1 mL of Reagent I (the proportion of tissue and Reagents can be kept constant), fully grinding on ice (using liquid nitrogen will have a better grinding effect)
Methylcobalamin acts as an essential cofactor in two primary enzyme reactions crucial for human metabolism: Homocysteine to Methionine Conversion: Methylcobalamin is required by the enzyme methionine synthase
Furthermore, additional data from 1567 subjects with the same measures of depression and metabolites repeated at 6-year follow-up (20102013) were used in a secondary analysis
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