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Cells were washed three times with ice-cold PBS and lysed in lysis buffer (40 mM Tris-HCl (pH 8.0), 1% Triton X-100, 1% NP-40, 10% glycerol, 0.15 M NaCl, 2 mM EDTA, 1 mM phenylmethanesulfonyl fluoride, 1 Complete protease inhibitor cocktail)
Beet Root (Beta vulgaris, 150mg) Providing naturally occurring betaine (trimethylglycine) as a methyl donor that supports normal liver methylation and healthy hepatic lipid metabolism, complementing the methionine and choline lipotropic activity.*
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The heart, skeletal muscle, and adipose tissues have an increased demand for carnitine during strenuous exercise or metabolic stress, at which point the liver and kidney adjust carnitine synthesis and reabsorption accordingly in response to the changing demands of these tissues
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