Using proper injection technique matters, including allowing the solution to reach room temperature before injecting and inserting the needle at the correct angle
Where direct clinical evidence is limited, mechanistic findings are interpreted as hypothesis-generating rather than causal, in order to avoid overextension of preclinical results
Primary antibodies were used as follows: HIF-1 (ab6489, 1:1000, Abcam, Cambridge, MA, USA), HIF-2 (ab207607, 1:1000, Abcam), ALKBH5 (ab195377, 1:1000, Abcam), SOX2 (ab97959, 1:1000, Abcam), NANOG (ab109250, 1:1000, Abcam), ALDH1 (#36671, 1:1000, CST), E-cadherin (sc-21791, 1:1000, Santa Cruz), GAPDH (ab9485, 1:5000, Abcam) and ACTIN (AC026 1:5000, Abcam)
"After 45 years, it starts decreasing and after 60 it is diminished," she added