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An equal volume of TrisHCl saturated phenol (pH 6.6) was added to the tube and further incubated on a shaker, at 125 rpm, for 10 min at room temperature

The remainder had a confirmed genetic cause including patients with contiguous deletions of TNXB and CYP21A2 , 3,6,7 whole TNXB gene deletions, and intragenic homozygous and compound heterozygous pathogenic variants

This distinction matters enormously for both safety and effectiveness