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Among the mutations considered, Glu113Lys enhanced catalytic performance and was added to X(GD_L)dX(GD_L)f to yield X(GD_L)gX(GD_L)i
This product is also prompted methylmalonic acid into succinic acid, involved in the citric acid cycle
Phiel et al
By establishing a complete traceability system, the entire process from raw materials to finished products can be traced, which is convenient for quickly locating the cause when problems occur
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