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Individuals who have had gastrointestinal surgery, such as weight loss surgery, may also have fewer cells through which to secrete stomach acid and intrinsic factor, inhibiting B12 absorption
this assigned the best hit for each of the ASVs and then provided the percentage of identical matches between the sequence of interest and the top hit as well as an e-value (i.e., the number of expected hits with a similar quality that could be found by chance)
Short-term storage at room temperature is acceptable for a few weeks without significant quality loss
A clinician then removes the needle, applies light pressure and disposes of the sharp safely in a sharps bin
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