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Approximately 1 g of peptide was injected and separated via liquid chromatography using a VanquishNeo UHPLC (Thermo, VN-S10-A-01) in Trap-and-Elute configuration using a PepMap Neo (5 M 300 M 5 mm) trap (Thermo, 174500) and an Aurora Frontier 60 cm 75 m C18 analytical column (IonOpticks, AUR3-60075C18) operating at a flow rate of 300 nl min 1 at a column temperature of 50 C
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In practice, once nightly usually means one injection in the evening each day, based on the schedule your clinician has selected
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