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For the orthotopic pancreatic cancer model, VRK2-WT or VRK2-KO Pan02 cells in logarithmic phase (8090% confluence) were harvested, detached with 0.25% trypsin-EDTA, centrifuged (1000 rpm, 5 min), and resuspended in cold serum-free RPMI-1640 medium

Photodynamic therapy Photodynamic therapy (PDT) is a cancer treatment that uses a drug and a certain type of light to kill cancer cells

Similarly, the frequency of PT-141 observed side effects are generally dose dependent, with an increased incidence of flushing, nausea, noxious aftertaste, post-nasal drip, headache, rhinorrhea, and vomiting at higher doses

adults affected, leading to annual healthcare costs of $173 billion