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Then, on the day following the second spinoculation, an equivalent to 2 10 4 cells was resuspended in 1.1 ml of MethoCult M3231 methylcellulose medium (Stem Cell Technologies Inc, Vancouver, Canada), supplemented with murine recombinant IL-3, IL-6, GM-CSF (each at 10 ng/mL), murine recombinant SCF (30 ng/mL, PeproTech, Rocky Hill, NJ), and G418 (1 mg/mL for selection)
Scientists are still unraveling why the immune system turns against the body
[12] Keam SJ
Due to the continuous exposure to occlusal forces and periodontal microorganisms, alveolar bone possesses a unique immune microenvironment distinct from the long bones, with more frequent interactions between monocytes/macrophages and MSCs ( Apart from the application in the treatment for periodontitis-related bone defects, NAC can also exert osteogenic function in dental implantology and orthodontics
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