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GHK was first identified in 1973 by Pickart as a factor in human plasma that altered the gene expression of cultured liver cells
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Western blot analysis RIPA Lysis Buffer (TransGen, Beijing, China) was used to extract total protein from tissues/fibroblasts and a Nuclear and Cytoplasmic Protein Extraction Kit (TransGen) was used to extract the protein from nucleus and cytoplasm of fibroblasts
10.1016/0028-3908(96)84626-0 34 GawrylukJ
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