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For the DDD-Ube2e2-COP1-c-Jun-STK40 complex, the purified protein was prepared at a concentration of 0.52 mg/ml and mixed with 0.02% N-Octyl--D-glucopyranoside (OG) prior to cryo-EM grids preparation to mitigate the effects of preferred orientation of the particles
This necessitates the development of small molecule inhibitors or mRNA delivery strategies to effectively harness its potential
SODIUM CHLORIDE, BENZOTRIAZOLYL DODECYL P-CRESOL
(PubMed) Joris PJ, Mensink RP
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