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High-performance liquid chromatography quantification of GSH/GSSG, 3-NT and 3-CT To evaluate biomarkers of oxidative stress and damage by high-performance liquid chromatography (HPLC), approximately 200 mg of tissue was minced and homogenized in 500 l of ice-cold phosphate-buffered saline
Some people respond strongly at 5 mg and never need to escalate
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In the INSPIRE cohort of 106 patients with advanced solid tumors treated with pembrolizumab (anti-PD-1 antibody) (NCT03702309) (235), higher serum choline levels were associated with augmented CD8 - T cells and B cells in the tumor stroma, as well as an elevated CD3 + CD8 + cytotoxic T cells to CD3 + CD8 - FOXP3 + T regulatory cells ratio in the tumors (235)
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