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As FITC fluorescence is pH-dependent, we analyzed the pH differences in the internalized FITC-agonist environment by measuring their fluorescence in cells treated with or without bafilomycin, a vacuolar-type H + ATPase which restores neutral endosomal pH 30
Immunohistochemical (IHC) stains of S-100 protein, MELAN-A and HMB-45 stains were positive and pancytokeratin stains were negative [Fig.4]
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