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The VEGFR2-Akt-eNOS angiogenic cascade increases blood vessel formation by 129-152% in preclinical studies
After blocking with blocking solution (0.2% casein, 0.3% Triton X-100, and 3% goat serum diluted in PBS) for 2 h at room temperature, anti-C3 (1:100 dilution) antibody-stained sections were reacted overnight at 4 C
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