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POMC neuron ablation was performed on 5-week-old iDTR:: Pomc -Cre mice by intracerebroventricular (i.c.v.) infusion of 2.5 ng of diphtheria toxin (DT) dissolved in 2.0 L of saline, followed by a 2-week period to ensure complete cell-lineage ablation [11-13]
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