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The skin is chronically exposed to both endogenous and environmental pro-oxidants leading to the production of ROS.[] ROS leads to lipid peroxidation, which is considered as the hallmark of oxidative stress in body.[] Malondialdehyde (MDA) is considered as the end product of lipid peroxidation and MDA levels is directly proportional to the oxidative stress.[] The most important antioxidants present in human body include superoxide dismutase, glutathione peroxidase, and catalase.[] Glutathione which is considered a master antioxidant can help keep the balance between the oxidant and antioxidant system in the body and prove to be an effective therapy but studies proving its efficacy are limited

The AUCell package (version 1.8.0) (19) utilizes a rank-based scoring approach to evaluate the activity levels of these pathways, generating a gene set activation score for each cell through the Area Under the Curve (AUC) function

In contrast, real-world settings introduce variability that is difficult to control