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The cells were then stimulated with 0.5 mM H 2 O 2 for 24 h, and the number of cells was determined with a Cell Counting Kit-8 (Dojindo) according to the manufacturers protocol
[33] Methods of assessment [edit] An increase in prothrombin time, a coagulation assay, has been used as an indicator of vitamin K status, but it lacks sufficient sensitivity and specificity for this application
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The binding of the GST-tagged protein to the DNA alters the migration of the DNA probe during gel electrophoresis, resulting in a shift in the DNA band's position
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