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Gene ontology analyses were performed using the DAVID functional annotation tool ( In vitro ubiquitylation assay Full-length FLAG-HA-OTUD5 was purified from 293 T cells treated with 10 M MLN7243 for 2 h before cell lysis, using anti-FLAG antibody (Sigma-Aldrich, M2) and was eluted using 200 ng/L of FLAG peptide (Sigma-Aldrich)

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Clinical data support meaningful outcomes at lower doses

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