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Li Z, Ferguson L, Deol KK, et al
Culture medium consisted of Dulbeccos Modified Eagle Medium (DMEM): Hams F-12 Nutrient Mixture (1:1) supplemented with 100 U/mL Penicillin and 100 g/mL Streptomycin, 2 mM Glutamine, 0.5x N2 supplement, 2.6 ng/mL insulin, 2 nM dexamethasone, 20 ng/mL epidermal growth factor (EGF), and 20 ng/mL basic fibroblast growth factor (bFGF)
In combination-therapy (Metformin + Berberine) the adverse events disappeared in one week after reduction in berberine dosage
Efficient nutrient uptake shows in higher motility and improved morphology on follow-up semen tests
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