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Freezing reconstituted peptides causes ice crystal formation that physically disrupts peptide structure, leading to aggregation and irreversible loss of bioactivity

This does two things: Keeps NAD+ precursors available for actual NAD+ synthesis Depletes the methyl groups that fat cells use to maintain their metabolic "off" state The second effect is the more interesting one

Minimum 15 neurons per treatment from 3 independent experiments were used for analysis