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However, under conditions of oxidative stress, superoxide radicals (O2) react with NO to form ONOO , reducing the bioavailability of NO and leading to endothelial dysfunction [67]

Many studies focus on the drugs ability to increase skin pigmentation but do not fully address long-term safety

After 24 h incubation, fibroblasts were fixed with 4% (w/v) paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with QuickBlock Blocking Buffer (Beyotime, Shanghai, China), and incubated with ASC antibody (1:400, Cell Signaling Technology, Danvers, MA, USA, 67824s) at 4 overnight

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