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A monoclonal antibody to -SMA and caspase-3 was used to incubate tissue sections, and the expression of these proteins was detected using the chromogen 3,3-diaminobenzidine tetrahydrochloride (DAB, Sigma-Aldrich ) stain
Numbness or tingling becomes constant rather than intermittent
Model 1030, Small Animal Instruments, Stony Brook, USA) and recorded with a data acquisition system (Acknowledge, Biopac Systems)
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