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Autophagy 17 (9), 20542081

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Ideally some of the cysteine is eventually converted to sulfate which is used as a conjugate for sulphation, a vital phase 2 detoxification pathway that handles many different toxins including excess oestrogen

Fmoc deprotection was achieved with 20% piperidine in DMF (twice for 2 min, room temperature, 300 rpm shaking) and peptide couplings were performed as double or triple couplings (twice for 5 min, 75 C, 300 rpm shaking, except for Arg and His, for which twice for 5 min, 50 C, 300 rpm shaking) consisting of AA/HCTU/DIPEA (ratio, 1:1.25:2.5) in fivefold excess compared to the resin