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Data was analyzed with QX Manager 1.2 (BioRad)
Following 4 and 8 weeks of treatment, oral glucose tolerance tests (OGTT) were performed
Enzyme-linked immunosorbent assay (ELISA) For the ligandreceptor affinity ELISA, the human FBXO22 protein served as an antigen to coat the 96-well plate at 4 C for 24 h
Next, to ask whether the effect of DIV-1 on germline proliferation is specific to GLP-1/Notch signaling, we depleted the expression of div-1 by RNAi in gld-3(q730) nos-3(q650) mutants with synthetic germline tumors
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